Composite

Part:BBa_K3748030:Design

Designed by: Sofía Jiménez Ochoa   Group: iGEM21_Groningen   (2021-10-01)


pREV1-αMF-BA-tTDH1


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1173
    Illegal XbaI site found at 1655
    Illegal SpeI site found at 1812
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1173
    Illegal SpeI site found at 1812
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal suffix found in sequence at 2436
    Illegal EcoRI site found at 1173
    Illegal BglII site found at 705
    Illegal BamHI site found at 1099
    Illegal XhoI site found at 954
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1173
    Illegal XbaI site found at 1655
    Illegal SpeI site found at 1812
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1173
    Illegal XbaI site found at 1655
    Illegal SpeI site found at 1812
    Illegal AgeI site found at 1737
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Golden Gate Assembly

Source

MoClo kit and Uniprot

References

Lee, M. E., Deloache, W. C., Cervantes, B., & Dueber, J. E. (2015). A Highly Characterized Yeast Toolkit for Modular, Multipart Assembly. ACS Synth. Biol., 4, 975–986.

https://www.uniprot.org/uniprot/P00692 - Alpha amylase B. amyloliquefaciens